47mark paper
PawSteps · Pets on the Green

GCSE Biology — Inheritance, Genetics & Variation

A full teaching unit taught through real animals — hedgehog colour morphs, bearded-dragon sex determination — with Punnett squares, worked examples, a mark scheme and a 47-mark exam paper.

AQA 4.6.1–4.6.2 · Edexcel Topic 3 · OCR B5 · WJEC Unit 2.4 · ~6–7 hours · Teach after Natural Selection · Prior knowledge: cells, DNA, variation

What you'll be able to do by the end

  1. Use the vocabulary of genetics precisely — gene, allele, genotype, phenotype, dominant, recessive, homozygous, heterozygous
  2. Explain the difference between sexual and asexual reproduction, and evaluate each
  3. Describe meiosis and explain why it produces genetic variation
  4. Complete and interpret a Punnett square
  5. Predict a monohybrid cross and express it as a ratio, fraction and percentage
  6. Explain how sex is determined in humans
  7. Describe inherited disorders — polydactyly and cystic fibrosis — and predict risk
  8. Discuss the ethical issues around embryo screening
  9. Explain the structure of DNA and what a gene does

Teach this after Natural Selection — that unit relied on "alleles are passed to offspring" without the mechanism. This is the mechanism.

The teaching below is free to read and print. The exam questions, mark scheme and teaching notes are PawSteps Premium.

Part 1 — The vocabulary

Get this right first. Almost every lost mark in genetics is a vocabulary error, not a reasoning error.

TermMeaning
GeneA short section of DNA that codes for a protein
AlleleA different version of the same gene
GenomeThe entire genetic material of an organism
ChromosomeA long molecule of DNA, carrying many genes
GenotypeThe alleles an organism has — written as letters, e.g. Bb
PhenotypeThe characteristic you can observe — e.g. brown eyes
DominantShows in the phenotype even with one copy. Capital letter
RecessiveOnly shows if two copies are present. Lower case
HomozygousTwo identical alleles — BB or bb
HeterozygousTwo different alleles — Bb
💡 The two that get confused

Genotype is the letters. Phenotype is what you see. A hedgehog with genotype Aa and one with AA can have exactly the same phenotype — "standard grey." Same phenotype, different genotype — that's the whole reason genetics is interesting, and what "recessive alleles hiding in a population" means.

⚠️ Gene versus allele

There is a gene for coat colour. There are several alleles of that gene — one for dark, one for pale, and so on. "The gene for brown eyes" is loose; it should be "the allele for brown eyes." Exam boards increasingly penalise this.

Part 2 — Sexual and asexual reproduction

SexualAsexual
ParentsTwoOne
CellsGametes fuse at fertilisationMitosis only
OffspringGenetically differentGenetically identical — clones
Cell divisionMeiosis to make gametesMitosis
VariationYesNo (except by mutation)

Sexual — advantages: produces variation; variation gives a survival advantage if the environment changes; natural selection can act, so the species can adapt; selective breeding is possible. Disadvantages: needs two parents; slower; more energy.

Asexual — advantages: only one parent; much faster; less energy; many identical offspring when conditions are favourable. Disadvantages: no variation, so the whole population is vulnerable to one disease or change; cannot adapt to a changing environment.

The organisms that do both

Malarial parasites reproduce asexually in the human host, sexually in the mosquito. Fungi use spores (asexual) when conditions are good, sexual reproduction when they deteriorate. Many plants use seeds (sexual) and runners (asexual). The pattern: asexual when conditions are good — make many copies fast; sexual when conditions are difficult or changing — make variation and hope some survive.

Part 3 — Meiosis

Meiosis makes gametes — sperm and egg in animals, pollen and egg in plants.

  1. The cell copies its genetic information — two copies of each chromosome
  2. The cell divides twice, producing four gametes
  3. Each gamete has half the number of chromosomes — a single set
  4. All four gametes are genetically different from each other

In humans, body cells have 46 chromosomes (23 pairs); gametes have 23. At fertilisation a sperm (23) fuses with an egg (23) to make a zygote with 46 — restored. If gametes had the full 46, every generation would double the number; halving in meiosis and doubling at fertilisation keep it constant.

MitosisMeiosis
PurposeGrowth, repair, asexual reproductionMaking gametes
DivisionsOneTwo
Cells produced24
Chromosome numberSame as parentHalf of parent
GeneticallyIdenticalAll different
💡 A memory hook

Mitosis makes more of the same. Meiosis makes mixed-up halves. Two divisions, four cells, half the chromosomes, all different.

Part 4 — Genetic crosses

A Punnett square predicts the possible genotypes of offspring from two parents.

Worked example — hedgehog coat colour

African pygmy hedgehogs come in many colour morphs. Here, standard grey (A) is dominant and albino (a) is recessive. Two heterozygous grey hedgehogs are bred: Aa × Aa. Each parent can pass on A or a:

Aa
AAAAa
aAaaa

Genotype ratio: 1 AA : 2 Aa : 1 aa. Phenotype ratio: 3 grey : 1 albino. Probability of albino: 1 in 4 = 25% = ¼.

⚠️ The mistake that costs marks every year

"One in four of the offspring will be albino." No — there is a 1 in 4 probability for each offspring. A litter of four could easily contain no albinos, or three. Write "a 25% chance" or "a probability of ¼." Never "one in four will be."

The three crosses you must know

Cross 1 — AA × aa: all offspring Aa — all show the dominant phenotype, all carry the recessive allele.
Cross 2 — Aa × Aa: 3 : 1 phenotype ratio; 25% chance of the recessive phenotype.
Cross 3 — Aa × aa: 1 : 1 phenotype ratio; 50% chance of the recessive phenotype.

The test cross

An organism showing the dominant phenotype could be AA or Aa — you can't tell by looking. Breed it with a homozygous recessive (aa): if any offspring show the recessive phenotype, the parent must be Aa; if all offspring show the dominant phenotype, it's probably AA. This is exactly what animal breeders do to find out whether an animal carries a recessive allele — including one for a disorder.

Part 5 — Sex determination

Humans have 23 pairs of chromosomes. 22 pairs control characteristics; the 23rd pair determines sex. Female: XX. Male: XY.

XX
XXXXX
YXYXY

Result: 2 XX : 2 XY — a 50% chance of each sex. The mother can only pass on X; the father passes X or Y, so the father's gamete determines the sex of the child.

Worth knowing

Sex determination differs across species. In birds, the female has the differing pair. In many reptiles — including bearded dragons and some tortoises — sex is determined by incubation temperature, not chromosomes. This has a real conservation consequence: rising temperatures are skewing sex ratios in wild turtle populations, with some beaches producing almost entirely female hatchlings.

Part 6 — Inherited disorders

Polydactyly — caused by a dominant allele

Extra fingers or toes. Because the allele (D) is dominant, only one copy is needed. A parent with polydactyly can pass it on even if the other parent is unaffected — Dd × dd:

dd
DDdDd
ddddd

50% chance of a child with polydactyly. Key point: a dominant disorder cannot skip a generation — if a child has it, at least one parent has it.

Cystic fibrosis — caused by a recessive allele

A disorder of cell membranes causing thick, sticky mucus in the lungs and digestive system. The allele (f) is recessive, so a person needs two copies to be affected. Carriers are heterozygous (Ff) — they have the allele but no symptoms. Two carriers, Ff × Ff:

Ff
FFFFf
fFfff

FF unaffected, not a carrier (25%) · Ff unaffected carrier (50%) · ff has cystic fibrosis (25%). A recessive disorder can skip generations — two unaffected carriers can have an affected child; 50% of children are carriers; neither parent has the disorder, which is why it can appear unexpectedly.

💡 Reading a family-tree question

If two unaffected parents have an affected child, the allele must be recessive — the parents are both carriers. If an affected child has two unaffected parents, it cannot be dominant. That single observation answers half of all pedigree questions.

Embryo screening

In IVF, embryos can be tested for disorder alleles before implantation; embryos without the disorder allele are implanted.

For: prevents suffering; reduces cost to the health service; laws prevent it going too far; parents already make choices about having children. Against: it implies people with genetic disorders are less valuable; it may lead to selecting non-medical characteristics; unused embryos are destroyed; it is expensive and unequally available.

Exam technique

This is an evaluate question and it will be levels-marked. To reach the top band you must give both sides and reach a judgement. There is no "right" opinion — the marks are for the quality of reasoning, not the conclusion.

Part 7 — DNA and the genome

DNA is a polymer made of two strands forming a double helix. Each strand is made of repeating units called nucleotides, each containing a sugar, a phosphate group, and one of four bases: A, C, G, T. Complementary base pairing: A pairs with T, and C pairs with G — always. So one strand determines the other, which is how DNA copies itself accurately.

What a gene does

A gene is a section of DNA that codes for a particular sequence of amino acids, which fold to make a specific protein. Three bases code for one amino acid. The order of bases determines the order of amino acids, which determines the protein's shape, which determines what it does.

The genome, and why sequencing it matters

The genome is the entire genetic material of an organism; the human genome was sequenced in 2003. It's useful to: 1. search for genes linked to disease; 2. understand and treat inherited disorders; 3. trace human migration patterns.

A connection worth making

The same technology classifies organisms into the three domains, identifies which species a piece of meat came from, and shows that archaea are as distinct from bacteria as we are. Genome sequencing links this unit to classification, evolution, disease and forensics — one technique underpinning several topics.

Exam-style questions

Answer all questions. Marks are shown in brackets. Total: 47 marks.

Question 1

In African pygmy hedgehogs, the allele for standard grey coat (A) is dominant to the allele for albino coat (a).

(a) A hedgehog has the genotype Aa. State its phenotype and explain your answer. (2)
Answer
(b) Two grey hedgehogs, both heterozygous, are bred. Complete a Punnett square and use it to state the probability that an offspring will be albino. (4)
Punnett square & answer
(c) A breeder has a grey hedgehog and wants to know whether it carries the albino allele. Describe a cross she could perform, and explain how she would interpret the results. (3)
Answer

(Total 9 marks)

Question 2

Cystic fibrosis is caused by a recessive allele (f).

(a) Explain what is meant by a carrier. (2)
Answer
(b) Two parents, neither of whom has cystic fibrosis, have a child who does. Explain how this is possible, using a genetic diagram. (4)
Genetic diagram & answer
(c) The parents have a second child. State the probability that this child will also have cystic fibrosis. Explain your answer. (2)
Answer
(d) Explain why cystic fibrosis can appear in a family with no previous history of it, but polydactyly cannot. (3)
Answer

(Total 11 marks)

Question 3

(a) Describe two differences between mitosis and meiosis. (2)
Answer
(b) Explain why gametes must contain half the normal number of chromosomes. (2)
Answer
(c) Strawberry plants can reproduce sexually by seeds and asexually by runners. Suggest one advantage of each method. (2)
Answer

(Total 6 marks)

Question 4

(a) State the sex chromosomes of a human male and a human female. (1)
Answer
(b) Use a genetic diagram to show why approximately half of all babies are female. (3)
Genetic diagram & answer
(c) In bearded dragons, sex can be determined by incubation temperature. Suggest one consequence for a wild population if average temperatures rise. (2)
Answer

(Total 6 marks)

Question 5

(a) Describe the structure of DNA. (4)
Answer
(b) Explain how the order of bases in a gene determines the protein that is made. (3)
Answer
(c) Give two uses of understanding the human genome. (2)
Answer

(Total 9 marks)

Question 6 — extended response

Embryos produced by IVF can be screened for alleles that cause inherited disorders; embryos carrying those alleles may not be implanted. Evaluate the use of embryo screening. (6)

Answer

(Total 6 marks)

TOTAL FOR PAPER: 47 MARKS

Mark scheme

Question 1

(a) 2. Standard grey1
A is dominant, so only one copy is needed for the characteristic to show1
(b) 4. Parental genotypes correctly identified as Aa × Aa1
Gametes correctly shown as A and a for each parent1
Punnett square correctly completed: AA, Aa, Aa, aa1
States 25% (accept ¼, 1 in 4, 0.25)1

Do not credit "one in four offspring will be albino" — the answer must be expressed as a probability.

(c) 3. Cross the grey hedgehog with an albino (aa)1
If any offspring are albino, the grey parent must be Aa / a carrier1
If all offspring are grey, the grey parent is probably AA1

Credit reference to needing many offspring — a small litter could produce no albinos by chance even from an Aa parent.

Question 2

(a) 2. A carrier is heterozygous — one copy of the recessive allele (Ff)1
No disorder themselves (one dominant allele is enough for normal function), but they can pass the allele on1
(b) 4. Both parents are carriers, genotype Ff1
Correct Punnett square: FF, Ff, Ff, ff1
Each parent passes on the f allele1
The child receives two recessive alleles (ff), so has cystic fibrosis1
(c) 2. 25% (or ¼)1
Each pregnancy is independent — the probability is not affected by the first child1

The second mark is the discriminator — many candidates assume the odds change.

(d) 3. Cystic fibrosis is recessive, so carriers show no symptoms and the allele can pass silently through generations1
It only appears when two carriers have a child and both pass on the recessive allele1
Polydactyly is dominant, so anyone with the allele shows the condition — it cannot be hidden, so cannot skip a generation1

Question 3

(a) 2 — any two: mitosis 2 cells / meiosis 4; mitosis identical / meiosis different; mitosis maintains chromosome number / meiosis halves it; mitosis one division / meiosis two.

(b) 2. At fertilisation two gametes fuse1
If gametes had the full number, the chromosome number would double each generation1
(c) 2. Sexual: produces variation, so some offspring may survive if conditions change or a disease appears1
Asexual: faster / needs no partner / many identical offspring quickly when conditions are good1

Question 4

(a) 1 — Male XY, female XX. Both required.

(b) 3. Parental genotypes: XX × XY1
Correct Punnett square showing XX, XX, XY, XY1
2 out of 4 are XX, so a 50% chance of female1
(c) 2. Rising temperature would produce a skewed sex ratio — mostly one sex1
Fewer breeding pairs / difficulty finding mates, so the population could decline or become locally extinct1

Question 5

(a) 4 — one each: a polymer / two strands; twisted into a double helix; repeating nucleotides (sugar, phosphate, base); four bases A/C/G/T with A–T and C–G pairing.

(b) 3. A sequence of three bases codes for one amino acid1
The order of bases determines the order of amino acids in the protein1
The amino acid order determines the protein's shape and therefore its function1

(c) 2 — any two: search for genes linked to disease; understand/treat inherited disorders; trace human migration.

Question 6 — extended response (6 marks, levels-marked)

Level 3 (5–6): balanced evaluation of both sides with explanation, reaching a justified conclusion. Level 2 (3–4): both sides with limited explanation, or one side thoroughly with brief acknowledgement of the other. Level 1 (1–2): relevant points, largely one-sided or listed.

Indicative — for: prevents a child being born with a serious disorder; reduces long-term cost; informed parental choice; regulation prevents misuse. Against: implies people with disorders are less valuable; risk of extending to non-medical traits; unused embryos destroyed; expensive/unequal; the disorder may vary in severity. Conclusion: any justified position — both are equally creditable; marks are for reasoning, not the view taken.

Common mistakes in this topic

"One in four will be albino." It's a 25% probability for each offspring, not a guaranteed outcome.

Assuming the odds change after an affected child. Each pregnancy is independent. Two affected children in a row is unlucky, not impossible.

Using "gene" where "allele" is meant. There's a gene for coat colour and several alleles of it.

Confusing genotype and phenotype. Genotype is the letters; phenotype is what you see.

Lower case for a dominant allele. Dominant is always a capital, recessive the same letter in lower case. B and r for the same gene is meaningless.

Forgetting to label the Punnett square. Gametes must be written on both edges; an unlabelled grid may not score.

One-sided answers to "evaluate." Both sides plus a judgement, every time.

Teaching notes

Vocabulary first, and drill it. Genotype/phenotype, gene/allele, homozygous/heterozygous. Students who muddle these lose marks on questions they understand. A five-minute recall starter every lesson for a fortnight beats any single explanation.

Punnett squares should be taught as a mechanical procedure. Parents' genotypes → gametes on the edges → fill the boxes → count. Students who improvise get lost; students with a fixed routine get full marks.

The probability point in Question 2(c) is the discriminator. Ask whether a couple who've had one affected child are more or less likely to have another — most say less. It's 25% every time, and the discussion sticks.

Hedgehog colour morphs work better than pea plants. Students who've seen a real albino hedgehog engage with aa in a way they don't with Mendel's peas — and colour morphs are a live topic among people who keep exotic pets.

Question 6 needs practising as a levels-marked question. Students trained on point-marked questions write a list and expect a mark each. Show them the level descriptors and mark two sample answers together.

Handle inherited disorders with care. Some students will have a relevant family history. Present them as conditions people live with, not tragedies to be prevented — particularly in the embryo-screening discussion, where a badly framed lesson can be genuinely hurtful.

Part of the Post-16 & GCSE resources · Adaptation & Competition → · Evolution & Classification →

Teaching is free · the exam paper & mark scheme are Premium